The amount of reactive nitrogen has more than doubled in terrestrial ecosystems due to human activities such fertiliser application that is predicted to increase dramatically in coming decades. We conducted a 3-year experiment in a Neotropical savanna in which we determined the effects of increased N deposition on litter decomposition […]
Quantitative stable isotope probing (qSIP) estimates the degree of incorporation of an isotope tracer into nucleic acids of metabolically active organisms and can be applied to microorganisms growing in complex communities, such as the microbiomes of soil or water. As such, qSIP has the potential to link microbial biodiversity and […]
Plant communities typically exhibit lagged responses to climate change due to poorly understood effects of colonization and local extinction. Here, we quantify rates of change in mean cold tolerances, and contributions of colonization and local extinction to those rates, recorded in plant macrofossil assemblages from North American hot deserts over […]
RNA is considered to be a short-lived molecule, indicative of cellular metabolic activity, whereas DNA is thought to turn over more slowly because living cells do not always grow and divide. To explore differences in the rates of synthesis of these nucleic acids, we used H218O quantitative stable isotope probing […]
Growing bacteria have a high concentration of ribosomes to ensure sufficient protein synthesis, which is necessary for genome replication and cellular division. To elucidate whether metabolic activity of soil microorganisms is coupled with growth, we investigated the relationship between rRNA and DNA synthesis in a soil bacterial community using quantitative […]
Permafrost is a distinct feature of the terrestrial Arctic and is vulnerable to climate warming. Permafrost degrades in different ways, including deepening of a seasonally unfrozen surface and localized but rapid development of deep thaw features. Pleistocene ice-rich permafrost with syngenetic ice-wedges, termed Yedoma deposits, are widespread in Siberia, Alaska, […]
The Arctic continues to warm at a rate that is currently twice as fast as the global average (see essay on Surface Air Temperature). Warming is causing normally frozen ground (permafrost) to thaw, exposing significant quantities of organic soil carbon to decomposition by soil microbes (Romanovsky et al. 2010, Romanovsky […]
The labeled atoms of carbon and nitrogen are widely used in biology, biochemistry, and soil science. Both radioactive (14C) and stable (12C, 13C,15N) isotopes are used as such labels. Recently, an increase in mass spec-trometry sensitivity made it possible to study natural ratios of carbon (13C/12C) and nitrogen (15N/14N) stableisotopes […]
For the past several decades, connecting biogeochemistry and microbial genomics has been a high priority in microbial ecology. Yet, techniques that actually link element flow and genomic information are scarce. In this project, we are using the Chip-SIP method to measure isotopic composition of major elements (C, N, H, and […]
The causes of the Pleistocene extinctions of large numbers of megafaunal species in the Northern Hemisphere remain unclear. A range of evidence points to human hunting, climate change, or a combination of both. Using ancient DNA and detailed paleoclimate data, Cooper et al . report a close relationship between Pleistocene megafau-nal extinction events and rapid warming events at the start of interstadial periods. […]