Search Results for: mo j

Using microbial communities and extracellular enzymes to link soil organic matter characteristics to greenhouse gas production in a tidal freshwater wetland

To gain a more mechanistic understanding of how soil organic matter (OM) characteristics can affect carbon mineralization in tidal freshwater wetlands, we conducted a long-term in situ field manipulation of OM type and monitored associated changes in carbon dioxide (CO2) and methane (CH4) production. In addition, we characterized microbial community […]

Subsampled open-reference clustering creates consistent, comprehensive OTU definitions and scales to billions of sequences

We present a performance-optimized algorithm, subsampled open-reference OTU picking, for assigning marker gene (e.g., 16S rRNA) sequences generated on next-generation sequencing platforms to operational taxonomic units (OTUs) for microbial community analysis. This algorithm provides benefits over de novo OTU picking (clustering can be performed largely in parallel, reducing runtime) and […]