Quantitative stable isotope probing (qSIP) estimates the degree of incorporation of an isotope tracer into nucleic acids of metabolically active organisms and can be applied to microorganisms growing in complex communities, such as the microbiomes of soil or water. As such, qSIP has the potential to link microbial biodiversity and […]
Microorganisms in soil assimilate, transform, and mineralize soil C to support growth. There are an estimated 2.6 × 1029 microbial cells containing 26 Pg C in soils worldwide. Consequently, quantifying microbial growth in soil is critical for determining the degree to which microorganisms contribute to the global C cycle. Measuring […]
The dynamics of soil phosphorus (P) control its bioavailability. Yet it remains a challenge to quantify soil P dynamics. Here we developed a soil P dynamics (SPD) model. We then assimilated eight data sets of 426-day changes in Hedley P fractions into the SPD model, to quantify the dynamics of […]
RNA is considered to be a short-lived molecule, indicative of cellular metabolic activity, whereas DNA is thought to turn over more slowly because living cells do not always grow and divide. To explore differences in the rates of synthesis of these nucleic acids, we used H218O quantitative stable isotope probing […]
Non-structural carbohydrates (NSCs), the stored products of photosynthesis, building blocks for growth and fuel for respiration, are central to plant metabolism, but their measurement is challenging. Differences in methods and procedures among laboratories can cause results to vary widely, limiting our ability to integrate and generalize patterns in plant carbon […]
Most soil bacterial taxa are thought to be dormant, or inactive, yet the extent to which they synthetize new rRNA is poorly understood. We analyzed 18O composition of RNA extracted from soil incubated with H218O and used quantitative stable isotope probing to characterize rRNA synthesis among microbial taxa. RNA was […]
Growing bacteria have a high concentration of ribosomes to ensure sufficient protein synthesis, which is necessary for genome replication and cellular division. To elucidate whether metabolic activity of soil microorganisms is coupled with growth, we investigated the relationship between rRNA and DNA synthesis in a soil bacterial community using quantitative […]
Ecooss is looking for a new postdoc. The postdoctoral research associate will conduct research at the interface between quantitative ecology and microbial genomics. The postdoc will use tools in bioinformatics, statistics, ecological modeling, as well as laboratory and field experiments, to develop new quantitative models describing how microorganisms grow and […]
Measuring the growth rates of individual bacteria taxa within a mixed sample gives a powerful view of the moving parts of a living ecosystem, especially when that sample is soil, where so many ecological processes occur. Ecoss research pioneered “qSIP” — a new method for gathering many simultaneous bacterial growth […]
Wildflowers splashed across a meadow in different sizes, shapes and colors offer more than just beauty. The natural mix of plant species in an ecosystem—its biodiversity—helps it grow faster and cycle nutrients more efficiently. These ecosystem functions also deliver life-sustaining services on which humans rely, such as purifying water and […]